PCR fingerprinting microbes polymorphic DNA by random amplification of

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The random amplification of polymorphic DNA for fingerprinting plants.

School of Biological Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom Molecular markers based upon DNA sequence variation have proved extremely effective tools for distinguishing between closely related genotypes. With the advent of PCR technology, methods have been developed that use amplified DNA sequences as molecular markers. These methods require very little template ...

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Differentiation of Iranian Strains of Brucella spp. by Random Amplification of Polymorphic DNA

101 isolates of Brucella spp. including the reference (n=7) and the Iranian field strains belonging to last years 1961-2003 (n=94) were biotyped and classified into 9 biotypes. Random amplified polymorphic DNA (RAPD) analysis using a ten mer-AP4 primer was generated 72 reproduciable DNA band. The typeability was 100%. The thirteen isolates were classified into five groups, each containing 2-4 b...

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Random amplified polymorphic DNA (RAPD) fingerprinting of Mycoplasma gallisepticum isolates from chickens

  Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...

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differentiation of iranian strains of brucella spp. by random amplification of polymorphic dna

101 isolates of brucella spp. including the reference (n=7) and the iranian field strains belonging to last years 1961-2003 (n=94) were biotyped and classified into 9 biotypes. random amplified polymorphic dna (rapd) analysis using a ten mer-ap4 primer was generated 72 reproduciable dna band. the typeability was 100%. the thirteen isolates were classified into five groups, each containing 2-4 b...

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ژورنال

عنوان ژورنال: Journal of Medical Microbiology

سال: 1993

ISSN: 0022-2615,1473-5644

DOI: 10.1099/00222615-39-3-161